Mammalian Cell Culture

Recombinant Protein Expression

• Generation of transient or stable mammalian cell lines for recombinant protein expression.
• Adaptation of cell lines to protein free medium.
• Adaptation of cell lines to growth in suspension.
• Scale up in roller bottles or stirred tanks or wavebag bioreactors.
• Development and storage of Working and Master Cell Banks.

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Protein Purification

• Product harvest to include tangential cross flow concentration
• Purification of tagged proteins using immobilised metal ion chromatography
• Quantification and purity assessment using SDS/PAGE and Western Blot analysis

• Custom Services
• Antibody Production
• Mammalian Cell Culture
  • Recombinant Protein Expression
  • Protein Purification
• PCR Genotyping