Mammalian Cell Culture
ARC BioServ offers a range of services resulting in the manufacture of mammalian cell secreted biomolecules including Upstream and Downstream Process scale-up and development.
Recombinant protein expression
- Stable and transient protein expression.
- Adaptation of cell lines to protein free medium.
- Adaptation of cell lines to growth in suspension.
- Scale up in roller bottles or stirred tanks.
- Development and storage of Working and Master Cell Banks.
Protein purification
- Product harvest to include tangential cross flow concentration
- Purification of tagged proteins using metal ion chromatography
- Quantification and purity assessment using SDS/PAGE and Western Blot analysis